FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3243.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3243.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5621031
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACATCAATCTCGTAT140990.25082587162390674TruSeq Adapter, Index 4 (97% over 37bp)
CTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCT81590.14515130765156783No Hit
CTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCT72870.1296381393377834No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC63750.11341335779859602No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG61550.10949948505887977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG21200.053.9838248
GTATGCC21800.052.3379547
CGTATGC22450.051.2902946
TCGTATG24250.047.62750645
ATGCCGT24350.046.71282649
AGTCACG30450.038.8511928
CTCGTAT29300.037.7463644
CACGGAC31300.037.46132331
TGCCGTC30750.037.33193650
CGGACAT31900.036.5353333
GTCACGG32350.036.1365929
GGACATC33550.034.6341934
CTGCTTG34350.033.31871459
GCCGTCT34400.033.16736251
ACGTCTG38900.031.48982215
CAGTCAC37700.031.4734927
ACGGACA37750.030.78221932
CCAGTCA40600.029.48558626
TGCTTGA39050.029.3981560
CGTCTGA42050.029.1316716
Produced by FastQC (version 0.11.5)