FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3240.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3240.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9742540
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTGAAGATCTCGTAT195170.20032763529839243TruSeq Adapter, Index 10 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG33300.048.7671148
GTATGCC33100.048.6390847
CGTATGC34200.047.07466546
TCGTATG38200.042.23700345
ATGCCGT40650.039.94944449
CTCGTAT47850.032.69498444
TGCCGTC51450.031.56355350
CTGCTTG51550.031.1638259
CACTGGT54100.030.4081231
ACTGGTG53950.030.3624432
CAGTCAC56150.029.79662727
GCCGTCT56650.028.4809451
CTGGTGA58300.028.39540733
TGCTTGA59600.027.1895160
ACGTCTG62750.027.10678915
AGTCACT61700.026.83259628
TCTCGTA60750.025.98292443
GATCTCG60200.024.99940941
CCAGTCA67350.024.84247426
CGTCTGA68700.024.81018416
Produced by FastQC (version 0.11.5)