FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3238.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3238.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2938127
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAATGTCATCTCGTAT29870.10166340665328626TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7050.039.71441348
CGTATGC7150.038.6694846
GTATGCC7350.038.56958447
TCGTATG7750.032.96616445
AGTCACG11750.030.38617928
ATGCCGT8950.030.1102949
GTCACGG12850.027.78502529
ACGTCTG14900.024.89856115
CCAGTCA16400.022.41127626
CAGTCAC16050.022.2453327
TGCCGTC12250.021.71324550
CGTCTGA17500.021.3993416
CACACGT17550.020.93952412
ACTCCAG18450.020.48961323
ACACGTC18000.020.41603713
GCACACG18400.020.16207711
GAACTCC20000.019.95142221
TCACGGA18800.019.1775530
ACGGAAT20350.018.74890332
CACGTCT20000.018.54942714
Produced by FastQC (version 0.11.5)