FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3237.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3237.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4254850
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGCAATATCTCGTAT65880.15483507056652995TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG12100.045.98919748
CGTATGC12350.045.05982646
GTATGCC12450.044.69790347
ACCACGC13800.040.32859832
TCGTATG14300.038.18105745
ATGCCGT15300.037.05677849
CCACGCA15700.035.8897433
CACGCAA16300.034.78335634
CGCAATA16950.033.44948236
ACGCAAT18400.031.38413435
CTCGTAT17500.030.9993844
GCAATAT18500.030.26895537
TGCCGTC20200.027.54798350
CTGCTTG18600.026.53235259
CACCACG21500.026.37435531
AGTCACC22250.025.8002728
GCCGTCT21100.025.54361351
CAGTCAC23600.024.76936527
ACGTCTG25550.024.24523715
GTCACCA25250.023.15050129
Produced by FastQC (version 0.11.5)