FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3236.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3236.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3580621
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCATTAATCTCGTAT43620.12182244364874138TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG8450.040.17580848
GTATGCC8700.039.02187347
CGTATGC9000.038.498946
GCATTAA9050.036.73928536
GGCATTA9500.036.47264535
ATGCCGT9900.034.64499349
TCGTATG10000.034.29902345
CCGGCAT10150.033.10250533
ACCGGCA10950.030.6857732
GTCACCG13650.025.89886929
CGGCATT13750.025.19928234
CTCGTAT12500.025.1992844
CACCGGC13700.024.78273831
TGCCGTC15450.023.10581650
TTAATCT14050.022.41897639
TCACCGG15500.022.35607530
CATTAAT15200.022.3348937
AGTCACC15850.022.3043828
ACGTCTG15900.021.79183215
CTGCTTG16000.021.65563259
Produced by FastQC (version 0.11.5)