FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3227.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3227.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7204445
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCGGTTATCTCGTAT97560.13541639918133874TruSeq Adapter, Index 27 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC17300.042.48408546
GTATGCC17500.041.9985547
TATGCCG18750.039.38530748
TCGTATG20550.035.08394645
ATGCCGT24400.029.97847449
CGGTTAT26750.029.30780637
TCGGTTA28600.027.90152236
ACCTTCG30550.026.57993732
CTCGTAT28200.026.3111544
ACGTCTG34350.024.45347415
CTTCGGT34900.023.06542834
AGTCACC35450.022.9066228
CCTTCGG35900.022.71540833
TGCCGTC32050.022.71372650
CAGTCAC36800.022.5423327
CTGCTTG32100.022.02477359
CACACGT39050.021.68984212
GGTTATC36300.021.40421938
CGTCTGA39150.021.27670116
GTTATCT35600.020.64535539
Produced by FastQC (version 0.11.5)