FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3222.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3222.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3951359
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTTGAACATCTCGTAT63640.16105851176772346TruSeq Adapter, Index 11 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC10350.047.34168646
GTATGCC11550.042.42307347
TATGCCG11700.041.57951748
TCGTATG12500.038.91892245
ATGCCGT13550.036.9357849
TCACGGT16100.030.65365230
TGCCGTC16450.030.21154850
CTCGTAT16400.029.45040544
AGTCACG18150.026.99854728
CAGTCAC20150.025.36102727
GTCACGG20350.024.42378229
GCCGTCT19800.024.39295651
CTGCTTG19300.023.39402459
TGCTTGA20200.022.35171560
TCTCGTA21600.022.03642743
ACGTCTG23350.021.73387315
CGGTTGA23250.020.92415233
CCAGTCA24500.020.85920526
CCGTCTT24700.019.41217252
CGTCTGA26400.019.22295416
Produced by FastQC (version 0.11.5)