FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3219.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3219.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6310484
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCATCGTATCTCGTAT75420.11951539691725706TruSeq Adapter, Index 1 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG15200.044.89947548
GTATGCC16350.043.45456747
CGTATGC16000.039.3740146
AGTCACG32000.035.54851528
ATCGTAT30900.034.9991237
CATCGTA32200.034.78173436
CGTATCT21750.033.3089839
TCGTATG16000.032.5929345
ATGCCGT18850.032.4920449
GTCACGT35700.031.47204229
TCGTATC34750.030.51673738
TCACGTC38000.029.65926630
CAGTCAC38400.029.53284827
TATCGCG9500.028.73612241
ACGTCTG42300.027.55206115
CGCGTAT8100.027.2215444
CGTCTGA42950.027.2165816
CCAGTCA42800.026.58001726
GTCATCG44100.025.95172934
GTATCGC10850.025.1602640
Produced by FastQC (version 0.11.5)