FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3216.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3216.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2927953
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTTGAATCTCGTAT30230.1032461928179858TruSeq Adapter, Index 5 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC6000.036.16567646
TATGCCG6750.033.18314748
GTCACCC10950.032.60463329
CACCCAG11150.032.33537331
GTATGCC7100.032.04137847
ACCCAGT11400.031.31814232
TCGTATG6750.028.5177445
AGTCACC14900.024.19603728
CAGTCAC14950.023.88139327
CACACGT17400.023.53303712
TCACCCA15500.023.48523530
ATGCCGT8850.022.93644149
ACGTCTG18350.022.88687715
CGTCTGA18650.022.33106816
CCCAGTT17250.021.70992333
GCACACG19100.021.62170611
TGCCGTC9650.021.39764650
ACACGTC19700.020.96317713
CCAGTCA17350.020.5793226
CTCGTAT9950.020.40145344
Produced by FastQC (version 0.11.5)