FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3215.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3215.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2454918
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACGGTTATCTCGTAT49680.20236928483965658TruSeq Adapter, Index 9 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6050.048.01443548
CGTATGC6050.047.43691346
GTATGCC6800.041.6902147
ATGCCGT7350.039.04591449
TCGTATG7750.037.48299845
ACGGTTA8850.033.2188936
CGGTTAT9450.029.6289437
TGCCGTC9850.028.7804750
CTCGTAT9950.027.43654644
AGTCACG11600.025.95084428
GAACGGT12000.025.08223734
GCCGTCT11800.024.61756951
ACGAACG12650.024.34924532
CGAACGG12650.024.07009733
GGTTATC11350.024.05230338
CACGAAC12700.023.9787531
CTGCTTG11850.021.85647859
CAGTCAC15400.021.3657527
AACGGTT14100.021.09836835
GTCACGA14600.020.85822929
Produced by FastQC (version 0.11.5)