FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3214.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3214.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16014945
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAAGGTTATCTCGTAT167760.1047521549402761TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC38700.034.81835646
GTATGCC40250.033.47752447
TATGCCG40900.032.7734248
TCGTATG43500.030.25220945
CCCAAGG50550.028.17939833
GTCACCC53150.026.53950329
ATGCCGT50050.026.50216949
ACCCAAG57250.024.7001532
CCAAGGT59350.024.00115834
AGGTTAT59750.022.96176337
AAGGTTA64550.022.17611936
CTCGTAT61450.021.13053544
AGTCACC70650.020.11455228
TGCCGTC67100.020.0809650
ACGTCTG75350.019.74040415
GGTTATC70000.019.04904438
CAGTCAC74800.018.95213127
CTGCTTG69550.018.51904759
CACACGT80850.018.2244812
GCACACG81900.018.07652511
Produced by FastQC (version 0.11.5)