FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3212.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3212.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2647026
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACACCTATCTCGTAT76390.28858802293592883TruSeq Adapter, Index 18 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG11650.058.28109448
CGTATGC12650.054.22928246
GTATGCC12850.053.1128747
ATGCCGT13850.049.02344549
TCGTATG14200.048.8028445
CGTACAC14350.048.29179833
ACGTACA14600.047.9470432
CACGTAC14800.047.06350731
TACACCT15350.044.91774435
GTACACC15650.044.05669834
ACACCTA15800.043.41692436
CTCGTAT16050.042.0872544
AGTCACG16450.041.70372828
TGCCGTC16750.041.37159350
CCTATCT15650.041.14862439
TCACGTA17200.040.69923430
GCCGTCT17550.039.28628551
GTCACGT18550.037.54860729
CAGTCAC19600.035.71632827
CTATCTC18150.035.48077440
Produced by FastQC (version 0.11.5)