FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3211.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3211.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2620460
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCGACTTATCTCGTAT58310.22251818383031988TruSeq Adapter, Index 12 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG9350.050.90598748
GTATGCC9450.050.7395847
CGACTTA9500.049.73475636
CGTATGC9900.048.78676246
ATGCCGT10550.045.77919849
TCGTATG11000.043.90808545
CTCGTAT13150.035.3984344
CACCTCG14300.034.51431331
GACTTAT13700.033.97667337
ACGTCTG15600.033.42744415
TGCCGTC14400.033.29657450
AGTCACC15000.032.90301528
GCACACG16600.031.83601211
CACACGT17000.030.67459712
CAGTCAC16000.030.62780827
ACCTCGA16550.029.60939832
CTGCTTG15800.029.24095259
GCCGTCT16750.028.62511851
CGTCTGA18350.028.41788516
CTCGACT17250.028.20171434
Produced by FastQC (version 0.11.5)