FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3207.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3207.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4276362
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCACTGATCTCGTAT53410.12489588112512458TruSeq Adapter, Index 27 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG9500.042.3662848
GTATGCC9600.041.9254547
CGTATGC10300.039.41594346
TCGTATG12300.033.29138645
ATGCCGT12350.033.15621649
TGCCGTC16450.025.10511250
CTCGTAT16850.023.05548344
GCCGTCT19850.020.6286851
CTGCTTG20350.020.46747259
CAGTCAC20650.020.1708327
AGTCACC20850.019.97687728
ACGTCTG22400.019.06175815
TGCTTGA22350.018.79253460
TCTCGTA20950.018.37637343
CCGTCTT23150.017.99045252
GATCTCG20650.017.96540341
CCTTCAC23550.017.68529333
GTCACCT23000.017.65293129
CCAGTCA23950.017.53831326
ATCTCGT22450.016.99265742
Produced by FastQC (version 0.11.5)