FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3204.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3204.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4350045
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCTTGCATCTCGTAT56380.12960785463138885TruSeq Adapter, Index 9 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC10750.043.95112647
CGTATGC10550.043.7891146
TATGCCG11250.041.37507648
TCGTATG12800.035.5449645
ATGCCGT14450.031.24363149
AGTCACG20300.028.62408328
CACGATC20700.027.73275831
CTCGTAT17950.026.51667844
ACGATCT21500.026.37522132
CTTGCAT20750.025.63776237
TCACGAT23300.025.5389230
TGCCGTC17950.025.34653350
GTCACGA23500.024.72576929
ATCTTGC22800.024.56064635
CAGTCAC24950.023.71022627
GCCGTCT18750.023.14515551
CGATCTT26000.021.9416333
CTGCTTG17900.021.70398959
ACGTCTG28800.021.14469515
CCAGTCA28600.020.92977726
Produced by FastQC (version 0.11.5)