FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3200.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3200.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3977875
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCCAATCTCGTAT69030.17353486472048518TruSeq Adapter, Index 23 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC11550.048.7870446
GTATGCC11900.048.23446347
TATGCCG12500.046.19862448
TCGTATG13100.041.94584345
ATGCCGT15650.037.3471349
TGCCGTC18100.031.51839450
CTCGTAT17700.030.05597944
GCCGTCT19200.028.80122451
CTGCTTG20800.026.24987859
AGTCACC21900.026.21058328
TGCTTGA22550.024.36795660
TCTCGTA22700.023.4357243
GTCACCA25250.023.42622229
CAGTCAC25200.023.33439627
ATCTCGT22950.022.87542342
CCGTCTT25050.022.63405652
CCAGTCA27150.021.7876426
ACTCCAA27500.021.25444836
CACCAAC27950.021.16375531
CAACTCC27400.020.94880734
Produced by FastQC (version 0.11.5)