FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3198.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3198.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3260115
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACACCATCTCGTAT68640.21054472004821914TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC12100.051.4869546
TATGCCG12400.050.24052448
GTATGCC12550.050.19856647
TCGTATG13900.044.0641945
ATGCCGT14400.043.99182549
TGCCGTC16950.037.99303450
CTCGTAT16550.036.58563644
AGACACC18050.033.93308635
CTGCTTG17900.033.63189359
GCCGTCT18600.033.1173551
ACACCAT17700.032.62670537
GACACCA19400.030.48930236
CAGTCAC21150.029.95463627
TCTCGTA20350.029.0659743
TGCTTGA21350.028.85297660
CCAGTCA23450.027.7629726
ACCATCT20000.027.64922339
CACCATC20450.027.38309538
AGTCACA23300.027.03994228
ACAAGAC23300.027.03994232
Produced by FastQC (version 0.11.5)