FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3196.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3196.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2757518
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTAAGCATCTCGTAT46560.1688474925639651TruSeq Adapter, Index 11 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC7900.051.83442746
GTATGCC8600.048.4292847
TATGCCG8900.046.7959848
AGCTAAG10250.043.36578834
GCTAAGC10450.042.87074735
TCGTATG9800.040.3563545
ATGCCGT10500.037.9985649
TAAGCAT11350.037.92945537
CTAAGCA12000.037.33327536
TGCCGTC11900.034.4104650
CTCGTAT11850.033.96557644
ACAGCTA13450.033.05007632
GTCACAG14150.030.919829
CAGCTAA15250.028.91798833
CAGTCAC16200.028.52054227
CTGCTTG14750.027.52538359
AAGCATC15600.027.37075838
TCACAGC16500.026.94032930
GCCGTCT15000.026.8323251
ACGTCTG18250.026.46523315
Produced by FastQC (version 0.11.5)