FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3194.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3194.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4073487
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATCTCATCTCGTAT140210.3442014176060952TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC21650.056.74271446
TATGCCG22100.056.0603648
GTATGCC22450.055.032547
TCGTATG23350.051.5623245
ATGCCGT24850.049.0114749
CACCGTA27050.046.84531831
CGTATCT28250.044.8499234
CTCGTAT26750.043.56937444
TGCCGTC28150.043.3902350
ACCGTAT29850.042.68460532
CCGTATC30450.041.72447233
GTCACCG31500.040.6704929
GCCGTCT29750.040.46843351
GTATCTC31400.040.4621135
TCACCGT31700.040.41389530
AGTCACC33200.038.58796728
CAGTCAC34850.037.46452327
CTGCTTG30500.037.0654259
TCTCGTA31650.034.6123843
CCAGTCA38150.034.59168226
Produced by FastQC (version 0.11.5)