FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3192.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3192.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5345350
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCATACATCTCGTAT127540.23859990458997074TruSeq Adapter, Index 10 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC19550.054.78167747
CGTATGC20300.053.10254746
TATGCCG20250.053.0603348
TCGTATG22900.046.1564245
AGCATAC25050.042.89509235
ATGCCGT25350.042.10934449
GCATACA25700.041.53781536
CAGCATA27500.039.8371834
CTCGTAT27750.037.7110744
CCAGCAT29450.037.0805533
TGCCGTC29350.036.25117550
CATACAT29300.035.12017437
CACCAGC32500.033.3868631
GCCGTCT31900.033.3533551
CTGCTTG31700.032.6817559
ATACATC31700.032.45971738
ACCAGCA34550.031.60849432
AGTCACC35650.030.1408728
CAGTCAC36800.029.95985227
TACATCT33650.029.95463839
Produced by FastQC (version 0.11.5)