FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3190.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3190.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5158930
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCTCGTAT110090.2133969640991446TruSeq Adapter, Index 11 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC18500.050.89055346
TATGCCG18650.050.6674348
GTATGCC18750.050.39867847
ATGCCGT20800.044.92539649
TCGTATG20950.043.9367945
GCTACCA22350.041.8117935
AGCTACC23950.039.3107934
CTCGTAT24000.036.89486744
TGCCGTC25950.036.14443650
ACAGCTA27900.033.87368432
CAGCTAC28950.033.0049533
CTACCAA28450.032.6008336
GCCGTCT29900.030.90129951
CTGCTTG29100.030.67048559
GTCACAG31100.030.49966229
TACCAAT30500.030.06536737
TCTCGTA29650.029.51018343
CACAGCT32550.029.03487231
TCACAGC33150.028.50797330
TGCTTGA32500.028.3234360
Produced by FastQC (version 0.11.5)