FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3183.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3183.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1087627
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCAAGTTATCTCGTAT28710.2639691732551693TruSeq Adapter, Index 6 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC4400.061.2489547
TATGCCG5050.054.05848748
CGTATGC4950.053.73645846
TCGTATG5450.050.0908945
ATGCCGT5600.048.1241849
TCACGGC6250.044.2412830
AGTCACG6750.040.96414628
TGCCGTC6550.040.60999750
CGGCAAG6800.039.63167633
GTCACGG7300.038.35727329
CTGCTTG6950.038.27272859
GGCAAGT6950.038.27272834
CAGTCAC7400.037.83893227
CCAGTCA7700.037.27551726
GCCGTCT7150.037.20216451
CACGGCA7350.037.1439331
CTCGTAT7200.035.97160744
ACGTCTG7900.035.88546415
GTTATCT7400.034.999439
TGCTTGA7900.033.6703160
Produced by FastQC (version 0.11.5)