FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3182.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3182.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2691704
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCGAACAATCTCGTAT92590.34398284506765975TruSeq Adapter, Index 12 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC13250.057.84893446
GTATGCC13900.055.1437747
TATGCCG14100.054.85600348
TCGTATG14700.051.9046545
ATGCCGT15800.048.51075449
TGCCGTC17550.044.27176750
CACCTCG18950.041.19030831
AGTCACC18950.041.1872528
CTCGTAT18200.040.57683644
GCCGTCT19200.040.10259251
ACCTCGA20150.039.7795532
CTGCTTG19000.039.42096759
CAGTCAC20450.038.50847227
TGCTTGA21500.035.16271660
CCAGTCA22500.035.00187726
GTCACCT22550.034.611929
CTCGAAC22700.034.53865434
CCGTCTT23450.032.98377652
ATCTCGT22250.032.5617342
ACGTCTG25200.032.49872615
Produced by FastQC (version 0.11.5)