FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3180.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3180.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5423280
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATAGCCATCTCGTAT127740.23554011594459442TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG22600.049.55494748
CGTATGC22700.049.3375546
GTATGCC23600.047.60433647
TCGTATG23550.046.66509645
CAATAGC28200.040.83271834
ATGCCGT27400.040.36286549
ATAGCCA27750.040.23362736
TAGCCAT26750.039.12090337
CTCGTAT27700.038.28391344
AATAGCC31050.037.8738235
CCAATAG30450.037.3557633
GCCGTCT31700.034.6669751
TGCCGTC32200.034.4547350
AGTCACC34100.033.66797328
ACCAATA34600.033.4852432
CTGCTTG32700.032.85973759
ACGTCTG38250.031.47606715
CAGTCAC37000.031.40840327
TGCTTGA34750.030.9212560
GTCACCA38250.030.38114429
Produced by FastQC (version 0.11.5)