FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3179.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3179.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3777487
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGAAGCAATCTCGTAT65740.1740310423305229TruSeq Adapter, Index 10 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC10100.049.89922746
GTATGCC10700.046.77404847
TATGCCG11100.045.403848
TCGTATG11750.042.59424245
ATGCCGT13900.036.0059249
CTCGTAT15200.031.3146844
TGCCGTC16700.029.75942650
GCCGTCT18800.026.24907151
CTGCTTG19100.024.92091459
AGCAATC19500.023.8709538
GCAATCT19750.023.74599539
AGTCACT21400.023.55212828
CAGTCAC21950.023.1223627
TCTCGTA20600.023.1059843
TGCTTGA21700.023.06400760
CCGTCTT22650.022.0963552
ACGTCTG24900.021.2244315
GTCACTG25200.020.27786629
CCAGTCA25350.019.88332226
AAGCAAT24750.019.79780437
Produced by FastQC (version 0.11.5)