FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3177.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3177.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3819694
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACTA15350.038.30547336
ACCACAG17050.035.30864332
ACTATCG12800.034.4520339
CTATCGG8150.032.20756540
CACAGAC18850.031.7500734
AGACTAT18150.031.62475437
CCACAGA18900.031.2957133
GACTATC18100.030.93824238
ACAGACT19500.030.5122535
TATCGCG5750.029.8255341
TATCGGG8750.029.19945341
AGTCACC22450.028.84284228
CACCACA23650.028.26733831
GTATGCC5050.027.72225447
CAGTCAC25250.025.92200527
CTATCGC6900.025.86874440
GTCACCA25950.025.2224329
ACGTCTG28300.024.61084615
TATGCCG5750.024.34705448
ACTCCAG29250.023.69434723
Produced by FastQC (version 0.11.5)