FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3176.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3176.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2800308
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTCGTAT63020.22504667343735046TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC10150.051.3784647
TATGCCG10350.050.3847448
CGTATGC10850.048.0637246
TCGTATG11500.045.04273245
CACCGCA13350.039.59160231
ATGCCGT13200.038.9759349
CTCGTAT13050.037.54726844
ACCGCAA15000.035.00316632
CCGCAAC15150.034.88453333
TGCCGTC15300.034.08379450
GCAACTA15800.033.6709335
TCACCGC15550.033.31372530
GTCACCG15950.032.4782729
CTGCTTG16050.031.83804959
CGCAACT16650.031.74178134
AGTCACC17250.030.03063228
GCCGTCT17300.029.73886351
CAGTCAC17700.029.66264327
TAATCTC15650.029.5197540
ACGTCTG18250.029.34145515
Produced by FastQC (version 0.11.5)