FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3174.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3174.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8395476
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGAGAAATCTCGTAT108220.12890275667514267TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG23550.047.1102748
CGTATGC23650.047.0601846
GTATGCC24550.045.19239447
ATGCCGT27900.037.8834949
TCGTATG27500.037.79908845
GTCACCG44050.030.98953629
CACCGAG44100.030.63746831
TGCCGTC36200.029.00413750
CAGTCAC51650.026.63354527
AGTCACC52000.026.52127828
CCAGTCA55750.024.92702726
GCCGTCT39700.024.68395451
CTCGTAT30650.024.20822544
ACCGAGA57100.024.0291632
CCGAGAG59200.023.11585833
TCACCGA60800.022.8551130
ACGTCTG64300.022.58834315
CTGCTTG37950.022.22700559
CGTCTGA68750.021.12638716
GAACTCC69800.020.81143421
Produced by FastQC (version 0.11.5)