FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3171.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3171.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5758406
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAAGGTGATCTCGTAT101580.17640298374237592TruSeq Adapter, Index 19 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG16600.049.54599448
GTATGCC17150.047.75339547
CGTATGC18050.045.3723446
CGTAAGG18300.045.1346133
ACGTAAG21250.038.87122332
ATGCCGT21500.038.416949
GTAAGGT21850.037.4811734
TCGTATG21450.037.3646345
AGTCACG25050.032.6968528
CACGTAA25450.032.18183531
TGCCGTC27550.030.10755550
TCACGTA28750.028.73190130
GTCACGT29450.027.81151229
GCCGTCT30550.027.38012751
CTCGTAT27300.027.30671544
CTGCTTG30150.026.58372359
TAAGGTG31250.026.20683535
GGTGATC26900.025.8912738
CAGTCAC31950.025.41755527
ACGTCTG36950.023.67980415
Produced by FastQC (version 0.11.5)