FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3170.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3170.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5528187
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCACCATCTCGTAT67450.12201106800475453TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG13400.044.40051348
GTATGCC13650.043.5873247
CGTATGC13900.042.8033746
TCGTATG16550.035.10378645
ATGCCGT17950.034.12066349
TGCCGTC23150.025.70051250
AGTCACG23550.025.56444528
CTCGTAT21800.025.52610244
GCCGTCT25100.023.00668751
CTGCTTG24800.022.4390859
CAGTCAC28850.021.47485427
GTCACGA29800.020.43769329
CACGAAT30950.019.45316331
TGCTTGA29500.019.21996560
CCAGTCA33100.019.03544426
ACGTCTG32700.018.83738115
CGTCTGA33300.018.70817416
CACACGT34650.017.87827712
TCTCGTA30600.017.72809243
CCGTCTT32800.017.71242752
Produced by FastQC (version 0.11.5)