FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3169.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3169.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4887347
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATGGCACATCTCGTAT106680.21827793279257643TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG14950.051.03476748
GTATGCC15650.049.42397347
CGTATGC15850.048.1378846
CTATGGC16800.046.66577533
TATGGCA19050.041.7052434
ACTATGG20150.039.0839432
TCGTATG19350.039.06902345
ATGCCGT19750.038.98579849
CACTATG20550.038.83415631
GTCACTA23000.035.30547729
GGCACAT21350.035.08129537
CTCGTAT21300.034.1777544
ATGGCAC23100.034.09025635
TGCCGTC24150.032.1726450
CTGCTTG26150.030.11507259
CAGTCAC28100.028.64860327
GCCGTCT27400.028.48427451
TGGCACA27050.028.3358736
ACGTCTG30200.027.23429915
GCACATC26450.026.9932638
Produced by FastQC (version 0.11.5)