FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3168.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3168.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2839231
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGAGATATCTCGTAT70700.2490110878614667TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG10700.056.5862848
CGTATGC10550.056.39759446
GTATGCC11150.053.99054347
TCGTATG12000.048.4162345
ATGCCGT13400.044.92338649
CACAGCG14250.042.73871631
CTCGTAT15150.038.81152744
TGCCGTC16450.036.1686150
CAGCGAG16900.035.82681733
GTCACAG17350.034.9006629
CTGCTTG16750.034.477359
ACGTCTG18600.034.0582315
ACAGCGA18450.033.57752632
GCCGTCT17350.033.4855551
TCACAGC18750.033.04144730
AGTCACA19050.032.33794428
CAGTCAC19200.032.0858727
CGTCTGA20250.031.28311516
CCAGTCA20350.030.61828626
CGAGATA20250.030.0727436
Produced by FastQC (version 0.11.5)