FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3167.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3167.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4079807
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGCTAGATCTCGTAT70680.17324348921407312TruSeq Adapter, Index 12 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG9900.046.66485648
GTATGCC10400.043.74883747
CGTATGC11150.040.49219546
GCTAGAT10850.039.67636537
TCGTATG12600.035.5546145
TGCTAGA13750.033.34456636
CTTGCTA13600.033.19764734
TTGCTAG14400.032.81162635
ACCTTGC13800.032.46529432
ATGCCGT13950.032.1134549
CTAGATC14600.028.04685438
TGCCGTC17450.026.2740550
TAGATCT16150.026.22189339
CTCGTAT17450.025.27153444
CCTTGCT17850.025.0973733
CACCTTG18250.024.93267631
CTGCTTG18350.024.60515459
GCACACG20400.023.6764211
ACGTCTG20200.023.39075315
GCCGTCT20200.022.35061551
Produced by FastQC (version 0.11.5)