FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3166.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3166.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2170389
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCGTAACATCTCGTAT45740.2107456313130964TruSeq Adapter, Index 11 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC7350.051.42824646
TATGCCG7400.050.1325148
GCGTAAC7500.049.46521435
GTATGCC7700.048.1815247
CTGCGTA8350.044.0106933
TCGTATG8850.041.9206445
TGCGTAA9150.041.6927734
CGTAACA8900.040.50443336
ATGCCGT9500.039.7873949
ACTGCGT9900.037.47710432
CACTGCG9900.037.124431
CTCGTAT10050.036.56693644
TAACATC10750.032.55643538
GCCGTCT11800.031.43903451
TGCCGTC11700.031.10948250
CTGCTTG11200.030.62551359
GTAACAT11600.029.86981437
CAGTCAC12550.029.56429327
TCTCGTA12350.028.90670243
ACGTCTG13900.027.19344715
Produced by FastQC (version 0.11.5)