FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3165.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3165.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3071008
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTAGGAGATCTCGTAT57490.1872023778511811TruSeq Adapter, Index 8 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC9350.049.03602246
GTATGCC9800.046.78436347
TATGCCG9950.045.37483648
CTAGGAG10950.042.50949535
TCGTATG11100.041.30511545
ATGCCGT12300.036.4211249
CTCTAGG12700.036.3763133
CACTCTA13700.034.23370731
CTCGTAT14350.031.46250744
ACTCTAG15300.030.4249532
TCTAGGA15800.029.46069734
TGCCGTC15350.029.41235550
TAGGAGA16250.027.78336336
CAGTCAC17550.027.72225627
CTGCTTG16950.026.63726659
CCAGTCA17900.026.59357826
ACGTCTG18300.026.58393315
GCCGTCT17050.025.86393451
GTCACTC18150.025.84073829
AGTCACT19400.024.71698828
Produced by FastQC (version 0.11.5)