FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3163.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3163.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2768371
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCTCGTAT43560.1573488524478836TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7650.049.41027548
GTATGCC7900.048.2896847
CGGTATA9000.047.83275233
GGTATAG9200.046.41248334
CGTATGC8300.045.11911846
GTATAGG10050.042.4870535
CACGGTA10550.041.13842831
TATAGGA10250.040.6336536
TAGGATC9700.038.96789638
ATGCCGT10050.036.2178149
ATAGGAT11500.035.91260537
TCGTATG10400.034.32588645
ACGGTAT12900.033.6442232
TCACGGT13150.032.73783530
AGTCACG13700.031.42411228
GTCACGG14400.030.62518329
TGCCGTC11850.029.83032250
CTCGTAT12300.029.02351444
CAGTCAC16600.026.14520527
GCCGTCT13000.025.5761551
Produced by FastQC (version 0.11.5)