FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3162.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3162.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3154224
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAACATCTCGTAT109470.34705842070823123TruSeq Adapter, Index 11 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG15700.056.39924648
CGTATGC15900.055.25045846
CGCCTTA16300.054.53791433
GTATGCC16250.054.27583347
CCTTAAC16800.053.33141335
GCCTTAA16700.053.23161334
TCGTATG17100.051.57790845
CTTAACA16950.051.00111436
ATGCCGT18150.048.40046349
CACGCCT19400.045.64630531
CTCGTAT19850.043.903444
TCACGCC20350.043.68808430
TTAACAT20400.042.37592737
GTCACGC20950.042.26980229
TAACATC20400.041.17498838
TGCCGTC21150.041.0387150
AGTCACG22650.038.942728
GCCGTCT23150.037.4932551
ACGCCTT23800.037.06042532
CTGCTTG23850.036.541359