FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3158.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3158.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4746630
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAACGCCATCTCGTAT58100.12240263091919952TruSeq Adapter, Index 1 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG11350.045.32864448
GTATGCC11350.045.02028347
TAACGCC13250.043.05675535
CGTATGC11800.042.7102146
ATAACGC15850.036.21463834
ATGCCGT14000.035.74861549
TCGTATG13350.035.39188845
CATAACG16450.034.68097333
ACGCCAT17750.031.15503737
AACGCCA20050.029.15222236
CTCGTAT17350.025.82032844
ACATAAC24000.024.93942532
CACATAA25400.024.25435331
TGCCGTC19350.024.2367950
TCACATA26300.023.15719230
GTCACAT26950.022.7285529
CAGTCAC27850.022.6229327
CGCCATC21550.022.08731338
AGTCACA27900.021.95463828
CTGCTTG20250.021.08671459
Produced by FastQC (version 0.11.5)