FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3156.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3156.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2848639
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGATTGATCTCGTAT61020.21420755666126876TruSeq Adapter, Index 20 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC9400.053.61602846
GTATGCC9650.051.86432347
TATGCCG9850.050.45503248
TCGTATG11450.044.0166545
ATGCCGT11750.041.70062349
AGTCACG13150.037.795328
CTCGTAT13150.035.93089344
GTCACGT15450.033.0749829
TCACGTC15600.032.98131630
TGCCGTC14850.032.75975850
CTGCTTG15600.031.63458459
CACGTCG15850.031.5778131
GCCGTCT16050.030.96461151
CAGTCAC16800.029.79268327
ACGTCTG18050.028.69702515
ACGTCGA17450.028.68242532
CCGTCTT17750.028.19616752
TCTCGTA17100.027.01704243
TGCTTGA18650.026.46109860
CGTCTGA19850.026.09477416
Produced by FastQC (version 0.11.5)