FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3151.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3151.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1623185
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCATTGCATCTCGTAT38280.23583263768455226TruSeq Adapter, Index 6 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6300.053.3311548
CGTATGC6150.052.35719346
GTATGCC6550.050.228547
TCGTATG6200.044.59632545
ATGCCGT7200.044.23430349
CTCGTAT7350.038.09485244
TGCCGTC7750.037.9339350
CAGTCAC11100.037.52445227
AGTCACT11750.036.04440728
CCATTGC11850.035.4426835
GTCACTC12200.033.85420229
ACTCCAT12450.033.4565732
CCAGTCA12800.032.54073726
CATTGCA12550.032.3502736
GCCGTCT8050.031.73783151
CACTCCA13300.031.58154731
TCACTCC13600.030.62657730
CTGCTTG8500.029.64767359
GCATCTC10450.029.47247740
GAACTCC14950.029.2657221
Produced by FastQC (version 0.11.5)