FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3149.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3149.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4502788
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTGCTTATCTCGTAT93190.20696066525894624TruSeq Adapter, Index 16 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC14950.049.3966847
TATGCCG14900.049.32699648
CGTATGC15450.048.0246146
GTGCTTA17750.043.97065736
TCGTATG17300.042.2821145
ATGCCGT18650.039.408749
CTCGTAT19200.037.36879344
TGCCGTC22100.032.78156750
CCAGTGC25700.030.64120733
AGTCACC25650.030.56686428
CACCAGT25700.030.50739531
CTGCTTG22250.030.20177859
ACCAGTG26200.030.05845532
GCCGTCT24500.029.85601651
CAGTCAC27150.029.3944227
TGCTTAT26800.028.99176437
AGTGCTT27050.028.85320535
GTCACCA27800.028.58027829
TCTCGTA25400.027.83390443
CAGTGCT28800.027.0999734
Produced by FastQC (version 0.11.5)