FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3148.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3148.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3217498
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTTGAATCTCGTAT40160.12481748240402947TruSeq Adapter, Index 18 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC8500.043.6462947
TATGCCG8600.041.9178748
CGTATGC8550.040.52560446
CACGTCC13550.039.26313431
ACGTCCT14300.037.44864332
AGTCACG15250.035.11577628
CGTCCTT17650.030.73600633
GTCACGT17550.030.5137129
ATGCCGT9850.030.20251549
TCGTATG10150.029.99947445
GTCCTTG18400.029.29296534
TCACGTC18550.028.86876530
CTCGTAT11650.027.93942544
CAGTCAC19550.027.929227
ACGTCTG21200.026.74481415
CGTCTGA22000.025.61318816
ACTCCAG22400.025.15776423
GAACTCC22800.025.02343621
CCAGTCA22450.024.63361526
GCACACG23750.024.02063211
Produced by FastQC (version 0.11.5)