FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3147.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3147.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4562082
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATACGATCTCGTAT115390.2529327618398792TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC17550.055.04127547
TATGCCG17650.054.7282348
CGTATGC18200.052.69091446
TCGTATG20300.046.89531345
ATGCCGT21100.045.44803649
TACGATC20750.040.81730738
TGCCGTC25450.037.9549450
CGAATAC27200.036.1571334
CTCGTAT25050.036.0469544
CTGCTTG26850.035.97788259
ATACGAT25600.035.27250337
GCCGTCT27600.034.99830651
ACGATCT24750.034.2205739
CCGAATA31250.031.91915333
CGATCTC27150.031.06663740
GTCACCG31600.030.9037929
AATACGA31850.030.10909536
TGCTTGA32600.029.73945660
AGTCACC33800.029.41040828
CCGTCTT33850.029.1566452
Produced by FastQC (version 0.11.5)