FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3146.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3146.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5338649
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACCACAATCTCGTAT80540.15086213759323755TruSeq Adapter, Index 18 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG14150.047.98495548
GTATGCC14900.046.0393947
CGTATGC14950.045.88541846
ACGTACC17550.040.68527632
TCGTATG17000.038.91102645
ATGCCGT17600.037.9822349
CGTACCA20450.034.5724433
CACGTAC20900.034.16427231
TACCACA22500.030.95583735
AGTCACG22900.030.87364428
CTCGTAT21900.030.3647244
GTACCAC23850.029.35037434
TGCCGTC23350.028.62900550
TCACGTA26050.027.4090830
GTCACGT27100.026.08879929
GCCGTCT25550.025.88967751
TCTCGTA27700.024.38559243
CTGCTTG25400.024.2519659
CCACAAT29900.023.7627637
CAGTCAC31050.023.4464927
Produced by FastQC (version 0.11.5)