FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3145.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3145.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6365315
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACACAGATCTCGTAT129070.20277079767458484TruSeq Adapter, Index 23 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC21300.052.08725446
TATGCCG21350.051.4726848
GTATGCC21650.050.76022747
TCGTATG24500.045.9982845
ATGCCGT27900.039.51403449
CTCGTAT29950.035.8751244
TGCCGTC33450.033.37640450
CTGCTTG35400.031.04550459
GCCGTCT36800.030.4331751
CAGTCAC38000.029.47559427
AGTCACC38200.028.954328
AACACAG39100.028.5548235
GATCTCG34450.028.5477841
TGCTTGA39300.028.23182760
TCTCGTA37350.028.20532643
CCAACAC40550.027.9653133
GTCACCA40000.027.65135629
CCGTCTT43100.025.90349852
CCAGTCA43700.025.79276726
ACGTCTG45300.025.7271115
Produced by FastQC (version 0.11.5)