FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3144.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3144.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4454026
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACGCTAATCTCGTAT87390.19620451250172316TruSeq Adapter, Index 4 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC15550.050.19108247
CGTATGC15650.049.64673646
ACGCTAA15850.049.0213836
GACGCTA16050.048.6285935
TATGCCG16050.048.19137648
TCGTATG17800.043.25683645
ATGCCGT17650.043.02958349
CGCTAAT18250.041.2322937
AGACGCT20700.038.38109634
CAGACGC21650.037.3435833
GCTAATC20550.034.74324838
CTCGTAT20950.034.24695644
ACAGACG23800.033.97086732
TGCCGTC22800.033.92419450
GTCACAG26150.031.05222929
GCCGTCT25100.030.53672851
CACAGAC26850.029.85227231
CTGCTTG24950.029.45816859
TAATCTC24150.029.12936640
CAGTCAC29250.028.36175527
Produced by FastQC (version 0.11.5)