FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3143.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3143.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3288852
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGCAATCTCGTAT71690.21797879624866062TruSeq Adapter, Index 10 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG10550.053.07802248
CGTATGC11900.048.23444746
GTATGCC12100.046.8586847
TCGTATG13300.042.89398245
ATGCCGT13000.042.26720449
TACCTGC14800.042.09385334
ACTACCT15150.041.81762332
CTACCTG15400.040.681133
CACTACC15850.039.52912531
GTCACTA17550.035.89953229
ACCTGCA18000.034.80494335
CTCGTAT17250.032.66609244
TGCCGTC17350.031.46823150
CCTGCAA19850.030.8558836
CTGCTTG16450.030.42638659
GCCGTCT17900.029.5237251
GCAATCT18900.029.44303139
CAGTCAC21550.029.39936427
TCACTAC22250.028.31626130
CCAGTCA22450.028.22119926