FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3140.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3140.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5078857
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACATCTCGTAT105070.20687725604402724TruSeq Adapter, Index 15 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG17450.051.7457248
CGTATGC18200.049.6143246
GTATGCC18900.047.59156847
TCGTATG19900.045.37590845
ATGCCGT20550.043.93979349
CTCGTAT23700.036.47600644
TGCCGTC26100.035.26674750
CTGCTTG26900.033.69915459
TCACAGG27950.033.6864230
CACAGGA28950.032.5234531
GTCACAG28950.031.79739829
GCCGTCT29800.030.77054651
CAGTCAC31100.029.8242727
TGCTTGA31350.029.02734660
ACACATC29950.028.74672738
TCTCGTA30200.028.27753643
AGTCACA33350.028.02204128
CCAGTCA35200.026.84892326
ACGTCTG35100.026.52364515
CCGTCTT34300.026.2234152
Produced by FastQC (version 0.11.5)