FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3139.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3139.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3704636
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTCCATATCTCGTAT83040.224151576565147TruSeq Adapter, Index 16 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG11450.049.82365848
CGTATGC11600.049.48176646
GTATGCC12250.046.57049647
TCGTATG13400.042.57377645
ATGCCGT13900.041.0417949
GTCACCC16100.035.65482729
CTCGTAT15650.035.3347544
TGCCGTC17750.032.3369450
CACCCTT17650.032.12747231
CTGCTTG18250.031.45184959
CCATATC19350.029.12046838
TCCATAT20500.028.51200737
GCCGTCT20500.028.34051351
TCACCCT20750.028.00208530
CAGTCAC20600.027.86615227
CATATCT19950.027.71836939
AGTCACC21050.027.27043728
ACCCTTC22000.026.251632
TCTCGTA21450.025.7803743
TGCTTGA22900.024.91250260
Produced by FastQC (version 0.11.5)