FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3138.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3138.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5134289
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAGTCAATCTCGTAT107140.2086754368521133TruSeq Adapter, Index 18 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG18150.050.1354248
GTATGCC18900.048.88710847
CGTATGC18950.048.2040546
ATGCCGT20750.044.0220649
TCGTATG20400.042.7190345
AGTCACG26800.035.6551628
TGCCGTC26350.034.26786850
CGTCAGT28200.034.0075133
GTCAGTC29050.033.9763234
GCCGTCT27100.033.1903551
ACGTCAG29100.032.35687332
CTCGTAT26050.032.24450744
GTCACGT30450.031.49617429
TCACGTC31600.030.12841630
CTGCTTG28900.028.94442659
CAGTCAC33800.028.89285727
CCGTCTT34000.026.96934152
GTCAATC33150.026.39395538
ACGTCTG39500.025.96107915
CCAGTCA38500.025.82101426
Produced by FastQC (version 0.11.5)